DIY Hyaluronic Acid Serum Recipe
DIY Hyaluronic Acid Serum is so easy to make and its ability to help your skin maintain moisture is unmatched.
Hyaluronic acid is all of the rave in anti-aging skincare. Unfortunately as we get older, our skin loses hyaluronic acid.
By helping skin stay hydrated, hyaluronic acid can temporarily plump and smooth the skin, which may make fine lines look less noticeable.

What is Hyaluronic Acid?
Contrary to the second part of its name, hyaluronic acid is not an actual “acid” per se. It will not burn your skin. Do not let the name scare you away because hyaluronic acid can be your skin’s best friend.
Hyaluronic acid is a natural glycosaminoglycan (polysaccharides that are an important component of connective tissues) that is naturally occurring in our bodies, with half of it being found in our skin.

It plays an integral role in maintaining and regulating moisture within our tissues. As we age the amount of hyaluronic acid production gradually slows down resulting in fine lines and wrinkles. Hyaluronic acid is a humectant, which means it helps attract and hold onto moisture. When used in skincare, it can help skin feel more hydrated and give it a temporarily smoother, plumper appearance. (source)
Can Hyaluronic Acid be Used as a Serum?
Hyaluronic acid, on its own cannot be used as a serum but all you need are a few ingredients to make your own DIY Hyaluronic Acid Serum.
Can I Use Hyaluronic Acid Every Day?

Hyaluronic acid serum is applied topically to damp skin before you apply your moisturizer. It can also be used in conjunction with other face creams such as sunscreen or your daily moisturizer.
I like to apply hyaluronic acid serum while my skin is still slightly damp, then follow with moisturizer. This gives the humectant some available moisture and the moisturizer helps reduce water loss from the skin afterward.
If you have sensitive or reactive skin, patch test a small amount first before using the serum all over your face.
Should You Use Hyaluronic Acid Morning or Night?
Used regularly as part of your skincare regimen, hyaluronic acid will help plump up your skin decreasing the appearance of fine lines and wrinkles. It is safe to use daily, but for people with the most sensitive skin, using in the morning and night may be a bit too much.
Hyaluronic acid basically acts like a sponge on your skin by attracting and retaining moisture due to its water-attracting properties. (source)
Should You Use Hyaluronic Acid & Vitamin C Serum Together?
These 2 powerhouse ingredients absolutely can be used together. For even better anti-aging results, you can whip up this DIY Vitamin C Serum Recipe and use it alongside your Hyaluronic Acid Serum.
How to Make DIY Hyaluronic Acid Serum?
It is no surprise that hyaluronic acid is a popular ingredient in the beauty industry with many beauty brands adding hyaluronic acid into their creams and serums.
The downside is that some brands only add a small amount of hyaluronic acid into their serums and some even mix it with toxic chemicals.
You can make your very own diy hyaluronic acid serum from home with the same great benefits of the more expensive brands.
To make your own hyaluronic acid serum, you will need to purchase some high quality Hyaluronic Acid Powder.
High or Lower Molecular Weight Hyaluronic Acid?
You'll find hyaluronic acid powders sold in different molecular-weight ranges. There isn't one universal cutoff for what counts as ‘low’ or ‘high,’ so those labels can vary from one supplier to another.
The two options I use for this recipe are approximately 800,000–1,000,000 Da and 1,000,000–1,500,000 Da. The first is the smaller of the two and can give the serum a somewhat lighter feel, while the larger molecular-weight option tends to create more of a hydrating film on the surface of the skin.
I wouldn't choose between these based on claims that one will penetrate deeply into your skin. For this DIY serum, both are primarily useful as humectants that help hydrate the skin and improve how it feels and looks.
Want to learn more about choosing ingredients for your own skincare formulas? Beauty Base is my ingredient reference library where you can compare ingredients, learn what they do, and find the information you need to formulate with more confidence.
DIY Hyaluronic Acid Serum Ingredients
You will need a few ingredients to make your own serum including:
Hydrosol or Floral Water - A few good options would be Lavender or Rose Hydrosol.
Distilled Water - If you don't have floral water, you can just add more distilled water.
Vegetable Glycerin - Vegetable glycerin is a humectant that helps draw water to the skin. We will be using our glycerin to help disperse the hyaluronic acid into our serum as well.
Xanthan Gum - Xanthan gum is a natural gum polysaccharide that is used as a thickener.
Hyaluronic Acid - I have chosen a high molecular weight hyaluronic acid for this recipe, but as we covered before, you can include both high and lower molecular weight options in your recipe. The hyaluronic acid powder used in this recipe may be listed by its INCI name, Sodium Hyaluronate, depending on the supplier.
Dermosoft Eco 1388 - Because this is water based, we will be adding a preservative.
Shelf life: Because this is a water-based serum, don't skip the preservative. This recipe uses Dermosoft 1388 Eco and is adjusted to approximately pH 5 to support preservative performance. Since homemade formulas aren't typically challenge tested, I don't give this serum a guaranteed shelf life. Make it with clean, sanitized equipment, keep it in a clean pump bottle, and discard it if you notice changes in the smell, color, texture, or appearance.
In the past I've recommended Geogard ECT as a broad spectrum preservative for water based products because it claims to be water soluble. But unfortunately the Benzyl Alcohol isn't completely water soluble which can lead to skin sensitization issues, which we don't want.
This formula calls for 1 gram of Dermosoft 1388 Eco.
I recommend purchasing a jewelry scale to accurately add preservatives to your formulations.
I have included a conversion as well, if you don't have a jewelry scale. You can add 3/16 teaspoon of the preservative Dermosoft 1388 Eco.
If you don't have measuring spoons this small, you can find them on Amazon here. Let's get to the recipe!
Hyaluronic Acid Serum Troubleshooting
Don't worry if your serum looks a little lumpy or uneven at first. Hyaluronic acid can take several hours to fully hydrate, so give it some time before deciding the texture isn't right.
White lumps or clumps: Hyaluronic acid and xanthan gum can take time to hydrate. Give the serum several hours and stir again. Thoroughly disperse the powders in glycerin before incorporating the mixture into the water phase, following the recipe’s existing method.
Thick or gel-like texture: A thick texture is not automatically a failure. Give the powders time to hydrate before judging the finished consistency.
Serum seems too thin: Viscosity may develop over time. Allow several hours for hydration and stir again before deciding the texture is too thin.
Sticky or tacky feel: Try using less serum and follow with moisturizer. Do not judge the formula by expecting tackiness to disappear within a fixed number of minutes.
When to discard: Discard the serum if you notice unexpected changes in odor, color, texture or appearance, or visible contamination.
DIY Hyaluronic Acid Serum
Materials
- 2 tablespoon Hydrosol (Rose hydrosol or Lavender hydrosol)
- 2 tablespoon Distilled water
- 3/16 teaspoon (1 gram) Dermosoft 1388 Eco Preservative
- ½ teaspoon Vegetable glycerin
- ⅛ teaspoon Xanthan gum
- ⅛ teaspoon Hyaluronic acid
Instructions
- Combine your distilled water and Dermosoft 1388 Eco in a glass beaker and mix until incorporated.
- Add your hydrosol to the water, preservative mixture.
- Add Glycerin, Xanthan Gum and Hylaluronic Acid to separate small beaker and mix well.
- Slowly incorporate the Glycerin, Xanthan Gum and Hyaluronic Acid mixture to water beaker, mixing well to incorporate. Set aside to swell.
- When blend is clear, test pH and adjust to a skin friendly pH of around 5
Notes
Preservative: Dermosoft 1388 Eco requires the pH to be > 7 when adding. That is why we add this to the water phase at the beginning. At the end adjust your pH to around 5 for best results. Learn all about adjusting the pH of your skincare products here.
Shelf life: Because this is a water-based serum, don't skip the preservative. This recipe uses Dermosoft 1388 Eco and is adjusted to approximately pH 5 to support preservative performance. Since homemade formulas aren't typically challenge tested, I don't give this serum a guaranteed shelf life. Make it with clean, sanitized equipment, keep it in a clean pump bottle, and discard it if you notice changes in the smell, color, texture, or appearance.
Tips: For best results apply to damp skin and follow up with moisturizer of choice.
Recommended Products
As an Amazon Associate and member of other affiliate programs, I earn from qualifying purchases.
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Dermosoft 1388 Eco Preservative
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Lavender Water by Leven Rose
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Rose Water by Leven Rose
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Hyaluronic Acid LMW 0.8 - 1.0 Million Daltons1g (0.035 oz) - $5.45
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Hyaluronic Acid HMW - 1.0 - 1.5 Million Daltons
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Xanthan Gum Clear
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Organic Vegetable Glycerin
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6PCS Glass Stirring Rod Stir Sticks
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Mini Stainless Steel Measuring Spoons
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Jeweler's Scale
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pH Indicator Strips 100/Box
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Glass Measuring Low Form Beaker Set 5
DIY Hyaluronic Acid Serum FAQs
How much hyaluronic acid powder should I use in a DIY serum?
Hyaluronic acid is effective at fairly low concentrations, and more isn't necessarily better. Too much can make a serum overly thick or sticky. This recipe uses an amount that gives you the hydrating benefits of HA while still creating a serum that's easy to use.
Does homemade hyaluronic acid serum need a preservative?
Yes. If your serum contains water or hydrosol, it needs an appropriate preservative. This recipe uses Dermosoft 1388 Eco and is adjusted to approximately pH 5 to support preservative performance.
Can I make hyaluronic acid serum with just water?
Hyaluronic acid can be hydrated in water, but once you make a water-based skincare product, microbial contamination becomes a concern. For a serum you plan to store and use repeatedly, I recommend following a properly formulated recipe that includes a preservative rather than simply mixing HA powder with water.
Why is my homemade hyaluronic acid serum sticky?
Some tackiness is normal with hyaluronic acid serums. Try using a smaller amount of serum and follow with your moisturizer. The molecular weight of the HA and the amount used can also affect how the finished serum feels.
Should I apply hyaluronic acid serum to damp skin?
You can apply hyaluronic acid to slightly damp skin, and that's how I like to use it. I follow it with moisturizer to help reduce water loss and keep my skin feeling hydrated.
Can hyaluronic acid dry out your skin?
Hyaluronic acid is a humectant used to help hydrate skin, but how a finished product feels can depend on the entire formula, your skin and your environment. If your skin feels dry or tight after using an HA serum, try applying it to slightly damp skin and following with a moisturizer.
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Hi Mindy, what preservative to you recommend for this recipe?
I usually just use it up and make more. But you could use Grapefruit Seed Extract which I buy here: https://simplepurebeauty.com/grapefruit-seed-extract. I have updated the recipe with instructions using this specific preservative. Hope that helps!! 🙂
Isn't that an antioxidant, and not actually a preservative?
Hi Tracey, Grapefruit Seed Extract (GSE) is a citrus seed based anti-microbial used as a preservative in skin care products. ... It is said to be anti-microbial, anti-septic, anti-bacterial, astringent and does also have some antioxidant activity. Use GSE at .2 to 1% to preserve most formulations.
its not a preservative. Its an antioxidant. You need a broad-spectrum preservative. Keep in the refrigerator and use up within two weeks.
Thanks Mindy! Sounds like a great recipe. I can't wait to try it!
I have tried to order these two products from Amazon but due to laws I can't get them shipped to here, any suggestions
From Australia
Netty here are some Aussie links for the products.
https://shop.newdirections.com.au/17-g-Hyaluronic-Acid-Powder
https://www.aussiesoapsupplies.com.au/glycerine-pf-vegetable.html
https://www.preciousorganics.com.au/search?q=Grapefruit+Seed+Extract
https://shop.newdirections.com.au/Herbs/Liquid-Extracts-Glycerine-Based/Grapefruit-Seed-Extract-Glycerine-Based
Hi Mindy
I have purchased the hyalurinic in the 1% solution (liquid) can I still use your recipe above or does it need to be adjusted
I have not tried this, but if it is just a liquid form of hyaluronic acid, you should be able to. I would probably use more of the liquid hyualuronic acid. Instead of a teaspoon, maybe try a tablespoon and see how it goes. Let me know how it goes!
Mindy, I found that the hyalouric powder formed white lumps. Have you experienced this and is there a way to avoid it? thank you.
I have not had issues with white lumps. Where did you get your hyaluronic acid powder?
I have white lumps too. I used this brand: https://amzn.to/2NkNIzx
Mine was lumpy at first, but after refrigeration, voila! No more lumps!!
Can you tell me if this hyaluronic serum can be used around the eyes?
Yes, there should be no issues with using this around the eyes. 🙂
Hi Mindy, I want to try this serum so bad but can't get the HA here in Canada. Is the Collagen powder the same thing? The ingredients say its Hydrolyzed Collagen 1 & 3 from bovine skin, from my research it sounds like the same stuff. I was just wondering if you knew for sure or if I just need to experiment.
I am a crusty ole fart, whom does not especially like to look unkept like many of my old buddies. I learned bout the Hyaluronic Acid Serum about a year ago. I tried it, WOW it actually works. The bags under my eyes have diminished, and my skin is smooth and looks great. I get a lot of people who say I look much younger than my 68yrs. I buy and make my own serum. Iv made mistakes in the past because I did not include any preservative. I use it every day right after a shower. On my face and on my back.My back used to get so dry and itchy in the winter months. I really dont want to look beautiful, but I do want to look pleasant and interesting. You can sit on your ass and complain or you can try this SIMPLE recipe, and look pleasant. Youll be glad you did.
Hi Mindy , Opps! I was so interested in having my say so about the Hyaluronic Serum, that I didnt bother to read the rest of the comments. I didnt know this was a lady's page . Sorry to butt in. But Im not embarrassed, I ment what I said. Its good stuff. Some of you older gals need to Quit using that stinky weird makeup, and be happy with who you are and your new smooth face. I would sure like it better. Thank You Mindy for the recipe with preservative.
What did you use as an emulsifier? If you don’t use a proper emulsifier since you are using water & oil ingredients, the serum won’t combine well so I just wanted to make sure I’m putting together these ingredients properly.
Hi Carson, the main ingredients are water, glycerin and your powder. They blend together quite well without an emulsifier.
Hi, Mindy!
I just made your amazing recipe for hyaluronic acid serum. It feels wonderful on my skin! Thank you! One question: Can I add vitamin C to this mixture (which includes the grapefruit seed extract)? I presume the liquid form of C would be best. Thank you very much for your response!
Hi There, As for the preservative, you can not use grapefruit extract as a preservative it is an antioxidant.
Hi there Diana, according to the research I've done Grapefruit Seed Extract (GSE) is a citrus seed based anti-microbial used as a preservative in skin care products. ... It is said to be anti-microbial, anti-septic, anti-bacterial, astringent and does also have some antioxidant activity.
Can you use rose water or purified water in place of distilled?
If you're using purified water, you should boil it first. If you purchased your rosewater, that should be fine to substitute. Here is a great article all about water and diy beauty recipes: https://crunchybetty.com/diy-101-working-with-water/
Hi Mindy, I just tried making the serum but the hyaluronic powder formed white lumps (I bought the one you recommended). How can I fix it? Also, which oil would you recommend adding in place of Frankincense? Thank you!
Hi Mindy. I am having so much fun and enjoyment from all the things I am reading about.
My BIG QUESTION IS: What is the difference between an extract and preservative oil? Please explain as I am having trouble figuring this on out to make the Hyaloronic Acid Serum. Thanks and have a blessed day.
I am not sure I quite understand the question. There are different types of preservatives out there. Some are better for products containing water, which is why I use the Grapefruit Seed Extract in this recipe. It does look like an oil in appearance so I guess it could be called a preservative oil as well.
I don't like a strong fragrance in the things I use on my face. So I have used the grape seed oil and lemon juice in my DUY Hyaluronic Acid Serum. Lemon Oil is acidic, should be able to use as a preservative, and it has a great aroma when I use it. What do you think?
Thanks so much, Mindy. I've been using it for about 6 months with a couple of changes. I use Manuka honey in place of the glycerin and swapped geranium oil for the lavender. My 60 year old skin has never felt better!
Hi dear plzz answer me. Can we add germall plus (preservative) in this HA serum instead of GSE? Somebody told me that germall plus is skin friendly preservative nd can increase the shelf life of HA serum upto 2 years . So plz tell me if you know about this preservative? Its pros nd cones? How much can i add if I follow your above recepie of making HA serum.
How serum should be used? Is it beneficial to apply on the face before applying my homemade anti aging cream?
Hems, yes, you would apply this to a damp face before applying your anti-aging cream. 🙂
One quick comment. It would help me to make a more consistent product if you could also include the grams measurements to your recipes. Thanks so much!
Hi Debra, that is definitely something I plan on doing moving forward. 🙂
How do you test the ph in this
Hi Jodi, you can check out the Checking the pH Tutorial of Skincare Products post here.
What if my recipe turned out a bit “jellyish”?
Hi Veronica, if your serum is too "jellyish" for your liking, you can try reducing the amount of xanthan gum you are using.